Koyunlarda Fasciola hepatica Enfeksiyonunun tespitinde Ekskresyon / Sekresyon Antijenleri Kullanılarak Dot-ELISA ve ELISA tekniklerinin Karşılaştırılması
نویسندگان
چکیده
منابع مشابه
Enfeksiyöz Bovine Rhinotracheitis Virusun Tespitinde IBRV Suş SD Rekombinant Glikoprotein D Kullanılarak İndirekt ELISA Yönteminin Geliştirilmesi ve Uygulanması
Glycoprotein D (gD) is the major structural protein of infectious bovine rhinotracheitis virus (IBRV). It can induce both humoral and cellular immunity thus it is a preferable protein for IBR diagnostic reagent. Regarding to DNAstar analysis, the major antigenic region of the gD fragment was amplified by PCR using the IBRV genomic DNA as a template, and subsequenly constructed into the recombin...
متن کاملEvaluation of Dot-ELISA Method Using Excretory-Secre- tary Antigens of Fasciola hepatica in Laboratory Diagno- sis of Human Fasciolosis
Fasciolosis diagnosis, due to low sensitivity of coprological diagnostic method has been challenging for a long period. In this study, Dot-ELISA, one of the simplest and the most sensitive tests in this regard, was evaluated using excretory-secretory antigens of Fasciola hepatica to diagnose human fasciolosis Three groups consisting of patients infected with fasciolosis (n= 95), patients with o...
متن کاملComparative diagnostic potentiality of ELISA and dot-ELISA in prepatent diagnosis of experimental Fasciola gigantica infection in cattle.
A glycoprotein (27 kDa) was isolated from crude somatic antigen of Fasciola gigantica by two steps affinity chromatography and was used in early detection of experimental fasciolosis in cattle by indirect ELISA and in dot-ELISA formats. Although, anti-27 kDa antibodies could be detected after 3 weeks post infection (WPI) by dot - ELISA which was one week later than indirect ELISA. The test, dot...
متن کاملThe value of micro-ELISA test in the diagnosis of Fasciola hepatica infection.
OBJECTIVE In sero-diagnosis of parasitic infection, it is essential to inspect cross-reactivity between the target parasite and other parasites in order to assess diagnostic performance. The aim of this study was to determine the cut-off value of antibody titer for diagnosis of F. hepatica (FH) infection by using the micro-ELISA and diagnostic performance of this test. METHODS The study popul...
متن کاملDesigning and evaluation of Dot-ELISA for diagnosis of Fasciola infection in cattle
Fasciolosis is a disease caused by liver fluck of the genus of fasciola. Diagnosis of fasciolosis has been challenging for a long period due to low sensitivity of the coprological diagnostic method. In this study, an in-house Dot-ELISA method; using excretion–secretory (ES Ag) and Crude (Cr Ag) antigens of fasciola was described for diagnosis of fasciolosis in cattle. For this...
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ژورنال
عنوان ژورنال: İstanbul Üniversitesi Veteriner Fakültesi Dergisi
سال: 2015
ISSN: 0250-2836
DOI: 10.16988/iuvfd.2015.14154